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My CartReactivity：Mouse,Rat,Monkey,Hamster,Human
Application：IF,IHC,WB,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5887
Host：Mouse
Qty：
Size：
| Name | Anti-RAD23B Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 5H1-A10-A7 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ICC 1:50-200. |
| Target | RAD23B |
| Background | The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011] |
| Swiss Prot | P54727 |
| Gene ID | 5887 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human RAD23B |
| Calculated MW | 43kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Monkey Hamster Human |
| Application | IF IHC ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with RAD23B antibody diluted at 1:1000;Lane: A431,K562,Jurkat,C6,NIH3T3,HeLa,SW480,CHO-K1,COS7.
Immunohistochemistry of paraffin-embedded Human prostate cancer with RAD23B antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using RAD23B antibody diluted at 1:100.
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