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My CartReactivity：Monkey,Human
Application：IF,WB,ICC,IP
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1956
Host：Mouse
Qty：
Size：
| Name | Anti-EGFR Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 6H11-2D11-G3 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | EGFR |
| Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Swiss Prot | P00533 |
| Gene ID | 1956 |
| Subcellular Location | Cell membrane Endoplasmic reticulum membrane Endosome Endosome membrane Golgi apparatus membrane Nucleus membrane Nucleus Secreted Single-pass type I membrane protein |
| Immunogen | Recombinant protein of human EGFR |
| Public Immunogen Range | 25-645aa |
| Calculated MW | 175kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Monkey Human |
| Application | ICC IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EGFR antibody diluted at 1:1000;Lane: COS7,A549.
Immunofluorescence analysis of HeLa cells using EGFR antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using EGFR antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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