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My CartReactivity：Mouse,Human
Application：ICC,IP,IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：6597
Host：Mouse
Qty：
Size：
| Name | Anti-SMARCA4 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 6D7-F7-B6 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | SMARCA4 |
| Background | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila.Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes.The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI,which is required for transcriptional activation of genes normally repressed by chromatin.In addition, this protein can bind BRCA1,as well as regulate the expression of the tumorigenic protein CD44.Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2.Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,May 2012] |
| Swiss Prot | P51532 |
| Gene ID | 6597 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human SMARCA4 |
| Calculated MW | 185kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | ICC IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SMARCA4 antibody diluted at 1:1000;Lane: K562,F9,HeLa.
Immunofluorescence analysis of HeLa cells using SMARCA4 antibody diluted at 1:100.
Immunoprecipitation analysis of K562 extracts using SMARCA4 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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