{{cart_num}}
My CartReactivity：Mouse,Rat,Monkey,Hamster,Human
Application：IF,ICC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：2237
Host：Mouse
Qty：
Size：
| Name | Anti-FEN1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 7H8-F4-C11 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
| Target | FEN1 |
| Background | The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate,thus passing the substrate from one enzyme to another.The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication.DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene.Therefore,secondary structure can deter the protective function of this protein,leading to site-specific trinucleotide expansions.[provided by RefSeq,Jul 2008] |
| Swiss Prot | P39748 |
| Gene ID | 2237 |
| Subcellular Location | Mitochondrion Nucleus nucleolus nucleoplasm |
| Immunogen | Recombinant protein of human FEN1 |
| Calculated MW | 36/43kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Monkey Hamster Human |
| Application | IF ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with FEN1 antibody diluted at 1:1000;Lane: CHO-K1,Raji,Raw264.7,C6,PC12,COS7,NIH3T3,HeLa,Jurkat.
Immunofluorescence analysis of HeLa cells using FEN1 antibody diluted at 1:100.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
