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My CartReactivity：Human
Application：IP,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：23411
Host：Mouse
Qty：
Size：
| Name | Anti-SIRT1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 2G10-F4-D9 |
| Dilution Ratio | WB 1:500-1000. IP 1:20. |
| Target | SIRT1 |
| Background | This gene encodes a member of the sirtuin family of proteins,homologs to the yeast Sir2 protein.Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes.The functions of human sirtuins have not yet been determined;however,yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.The protein encoded by this gene is included in class I of the sirtuin family.Alternative splicing results in multiple transcript variants.[provided by RefSeq,Dec 2008] |
| Swiss Prot | Q96EB6 |
| Gene ID | 23411 |
| Subcellular Location | Cytoplasm Mitochondrion Nucleus Nucleus PML body |
| Immunogen | Recombinant protein of human SIRT1 |
| Calculated MW | 61/82kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SIRT1 antibody diluted at 1:1000;Lane: CEM,A549,Ramos,Jurkat,HeLa.
Immunoprecipitation analysis of HeLa extracts using SIRT1 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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