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My CartReactivity：Rat,Mouse,Human
Application：ICC,IP,IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1869
Host：Mouse
Qty：
Size：
| Name | Anti-E2F1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 4G8-2C2-C12 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | E2F1 |
| Background | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. |
| Swiss Prot | Q01094 |
| Gene ID | 1869 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human E2F1 |
| Calculated MW | 47kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ICC IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with E2F1 antibody diluted at 1:1000;Lane: C6,Raw264.7,HeLa.
Immunofluorescence analysis of HeLa cells using E2F1 antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using E2F1 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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