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My CartReactivity：Human
Application：IP,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5777
Host：Mouse
Qty：
Size：
| Name | Anti-PTPN6 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2a |
| Clone Number | 2B7-H7-F8 |
| Dilution Ratio | WB 1:500-1000. IP 1:20. |
| Target | PTPN6 |
| Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Swiss Prot | P29350 |
| Gene ID | 5777 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human PTPN6 |
| Calculated MW | 68kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PTPN6 antibody diluted at 1:1000;Lane: Human tonsil,Daudi,Jurkat,Raji.
Immunoprecipitation analysis of Raji extracts using PTPN6 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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