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My CartReactivity：Rat,Mouse,Human
Application：IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2932
Host：Mouse
Qty：
Size：
| Name | Anti-GSK3B Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 1A6 |
| Dilution Ratio | WB 1:1000-2000. IHC 1:100-200. |
| Target | GSK3B |
| Background | In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. |
| Swiss Prot | P49841 |
| Gene ID | 2932 |
| Subcellular Location | Cell membrane Cytoplasm Nucleus |
| Immunogen | Synthetic Peptide |
| Calculated MW | 47kDa |
| Purification Method | Saturated ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GSK3�� antibody diluted at 1:1000, lane1-3: Hela,3T3 Cell, Rat brain tissue
Immunohistochemistry of paraffin-embedded Human Breast Carcinoma with GSK3B antibody diluted at 1:200.
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