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My CartReactivity：Mouse,Rat,Human
Application：WB,IHC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2026
Host：Mouse
Qty：
Size：
| Name | Anti-ENO2 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 1.30E+03 |
| Dilution Ratio | WB 1:1000-5000. IHC 1:200. |
| Target | ENO2 |
| Background | Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. |
| Swiss Prot | P09104 |
| Gene ID | 2026 |
| Subcellular Location | Cell membrane Cytoplasm |
| Immunogen | Synthetic Peptide |
| Calculated MW | 47kDa |
| Purification Method | Saturated ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ENO2 antibody diluted at 1:3000 ,lane: Hela, Jurkat, 293T cell
Immunohistochemistry of paraffin-embedded Human prostate cancer with ENO2 antibody diluted at 1:200
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