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My CartReactivity：Rat,Mouse,Human
Application：IHC,IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：3875
Host：Mouse
Qty：
Size：
| Name | Anti-Cytokeratin 18 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 1B3 |
| Dilution Ratio | WB 1:1000-3000. IF 1:100-200. IHC 1:200-500. |
| Target | KRT18 |
| Background | Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. |
| Swiss Prot | P05783 |
| Gene ID | 3875 |
| Subcellular Location | Cytoplasm Nucleus nucleolus perinuclear region |
| Immunogen | Recombinant Protein |
| Calculated MW | 48kDa |
| Purification Method | Saturated ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IHC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Cytokeratin 18 antibody diluted at 1:2000;Lane: HepG2,HeLa,Mouse liver,C2C12,Rat heart,Mouse skeletal muscle.
Immunohistochemistry of paraffin-embedded Human breast cancer with Cytokeratin 18 antibody diluted at 1:500
Immunofluorescence analysis of HeLa using Cytokeratin 18 antibody diluted at 1:100.
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