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My CartReactivity：Mouse,Human,Rat
Application：WB,IHC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：4846
Host：Mouse
Qty：
Size：
| Name | Anti-NOS3 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | Mix |
| Dilution Ratio | WB 1:1000-2000. IHC 1:200-500. |
| Target | NOS3 |
| Background | Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), generates NO in blood vessels and is involved with regulating vascular function. |
| Swiss Prot | P29474 |
| Gene ID | 4846 |
| Subcellular Location | Cell membrane Cytoplasm Golgi apparatus Membrane caveola cytoskeleton |
| Immunogen | Recombinant Protein |
| Calculated MW | 67/68/133kDa |
| Purification Method | Saturated ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human Rat |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NOS3 antibody diluted at 1:1000 ,lane: Rat Heart Tissue
Immunohistochemistry of paraffin-embedded Mouse Colon Tissue with NOS3 antibody diluted at 1:500
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