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My CartReactivity：Human
Application：IF,ICC,IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5499
Host：Mouse
Qty：
Size：
| Name | Anti-PPP1CA Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 1C11-C10-H5-E9 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ICC 1:50-200. |
| Target | PPP1CA |
| Background | The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Swiss Prot | P62136 |
| Gene ID | 5499 |
| Subcellular Location | Cytoplasm Nucleus nucleolus nucleoplasm |
| Immunogen | Recombinant protein of human PPP1CA |
| Calculated MW | 33/38/39kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PPP1CA antibody diluted at 1:1000;Lane: A431,Jurkat,HeLa.
Immunohistochemistry of paraffin-embedded Human breast cancer with PPP1CA antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using PPP1CA antibody diluted at 1:100.
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