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My CartReactivity：Rat,Human
Application：WB,IF,IHC,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3309
Host：Mouse
Qty：
Size：
| Name | Anti-HSPA5(C-term) Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 9E4-2A7-H6 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ICC 1:50-200. |
| Target | HSPA5(C-term) |
| Background | The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Swiss Prot | P11021 |
| Gene ID | 3309 |
| Subcellular Location | Cytoplasm Endoplasmic reticulum lumen Melanosome |
| Immunogen | Recombinant protein of human HSPA5(C-term) |
| Calculated MW | 72kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human |
| Application | ICC IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HSPA5(C-term) antibody diluted at 1:1000;Lane: LNCaP,C6,MDA-MB-468,HeLa.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with HSPA5(C-term) antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using HSPA5(C-term) antibody diluted at 1:100.
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