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My CartReactivity：Mouse,Monkey,Human
Application：IF,FCM,ICC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：47
Host：Mouse
Qty：
Size：
| Name | Anti-ACLY Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2a |
| Clone Number | 3D9-E9-H8 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. FCM 1:50-100. ICC 1:50-200. |
| Target | ACLY |
| Background | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Swiss Prot | P53396 |
| Gene ID | 47 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human ACLY |
| Calculated MW | 91/121kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Monkey Human |
| Application | IF FCM ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ACLY antibody diluted at 1:1000;Lane: HeLa,K562,COS7,NIH3T3.
Immunofluorescence analysis of HeLa cells using ACLY antibody diluted at 1:100.
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