English Name |
Anti-CDK5 Monoclonal Antibody |
Chinese Name |
细胞周期依赖性蛋白激酶5抗体 |
Host |
Mouse |
Clone Type |
Monoclonal Antibody |
Subtype |
IgG1 |
Clone Number |
2E8-F9-B7-C11 |
Dilution Ratio |
WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
Target |
CDK5 |
Synonyms |
Cdk 5, Cell division protein kinase 5, Crk6, Cyclin dependent kinase 5, Protein kinase CDK5 splicing, PSSALRE, Serine threonine protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, CDK5_HUMAN |
Background |
Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. |
Swiss Prot |
Q00535 |
Gene ID |
1020 |
Subcellular Location |
Cell junction Cell membrane Cell projection Cytoplasm Nucleus Perikaryon Peripheral membrane protein growth cone lamellipodium postsynaptic cell membrane postsynaptic density synapse |
Immunogen |
Recombinant protein of human CDK5(N-term) |
Calculated MW |
33kDa |
Purification Method |
Affinity purification |
Storage Buffer |
PBS with 0.02% sodium azide,0.5% BSA,50% glycerol,pH7.3. |
Storage |
Store at -20℃. Avoid freeze / thaw cycles. |
Unit |
Piece |
Specification |
50ul 100ul |