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My CartReactivity：Mouse,Rat,Monkey,Human
Application：IF,ICC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1020
Host：Mouse
Qty：
Size：
| Name | Anti-CDK5 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 2E8-F9-B7-C11 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. ICC 1:50-200. |
| Target | CDK5 |
| Background | Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. |
| Swiss Prot | Q00535 |
| Gene ID | 1020 |
| Subcellular Location | Cell junction Cell membrane Cell projection Cytoplasm Nucleus Perikaryon Peripheral membrane protein growth cone lamellipodium postsynaptic cell membrane postsynaptic density synapse |
| Immunogen | Recombinant protein of human CDK5(N-term) |
| Calculated MW | 33kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Monkey Human |
| Application | IF ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CDK5 antibody diluted at 1:1000;Lane: NIH3T3,HeLa,PC12,COS7,Jurkat,K562.
Immunofluorescence analysis of HeLa cells using CDK5 antibody diluted at 1:100.
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