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My CartReactivity：Human
Application：IP,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：695
Host：Mouse
Qty：
Size：
| Name | Anti-BTK Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 5B12-C9-F10 |
| Dilution Ratio | WB 1:500-1000. IP 1:20. |
| Target | BTK |
| Background | The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Swiss Prot | Q06187 |
| Gene ID | 695 |
| Subcellular Location | Cell membrane Cytoplasm Nucleus Peripheral membrane protein |
| Immunogen | Recombinant protein of human BTK |
| Calculated MW | 76/80kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IP WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with BTK antibody diluted at 1:1000;Lane: Daudi,K562,Ramos,Raji.
Immunoprecipitation analysis of Raji extracts using BTK antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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