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My CartReactivity：Rat,Mouse,Human
Application： IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-HSPA9 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. IF 1:50-200. |
| Target | HSPA9 |
| Background | This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2. |
| Swiss Prot | P38646 |
| Gene ID | 3313 |
| Subcellular Location | Mitochondrion Nucleus nucleolus |
| Immunogen | Recombinant protein of human HSPA9 |
| Public Immunogen Range | 380-679aa |
| Calculated MW | 74kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded mouse heart tissue using HSPA9 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U2OS cell using HSPA9 antibody. Blue: DAPI for nuclear staining.
Western blot analysis with HSPA9 antibody diluted at 1:1000;Lane: MCF7 NIH3T3 COS-7 Mouse brain Mouse lung.
Immunohistochemistry of paraffin-embedded rat kidney with HSPA9 antibody diluted at 1:100.
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