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My CartReactivity：Rat,Human,Mouse
Application：IF,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2572
Host：Rabbit
Qty：
Size：
| Name | Anti-GAD2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. |
| Target | GAD2 |
| Background | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Swiss Prot | Q05329 |
| Gene ID | 2572 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human GAD2 |
| Public Immunogen Range | 401-585aa |
| Calculated MW | 65kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunofluorescence analysis of A549 cell using GAD2 antibody. Blue: DAPI for nuclear staining.
Western blot analysis with GAD2 antibody diluted at 1:1000;Lane: U-87MG Mouse brain Rat brain.
Immunofluorescence analysis of rat brain cells using GAD2 antibody diluted at 1:50.
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