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My CartReactivity：Mouse,Rat,Human
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：382
Host：Rabbit
Qty：
Size：
| Name | Anti-ARF6 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IF 1:50-200 |
| Target | ARF6 |
| Background | This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. |
| Swiss Prot | P62330 |
| Gene ID | 382 |
| Subcellular Location | Cell membrane Cell projection Cleavage furrow Cytoplasm Endosome membrane Golgi apparatus Lipid-anchor Midbody Recycling endosome membrane filopodium membrane |
| Immunogen | Recombinant protein of human ARF6 |
| Public Immunogen Range | 1-175aa |
| Calculated MW | 20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-2000. IF 1:50-200. |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ARF6 antibody diluted at 1:1000
Immunofluorescence analysis of HeLa cells using ARF6 antibody diluted at 1:100.
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