English Name |
Anti-SFN Polyclonal Antibody |
Chinese Name |
分层蛋白抗体 |
Target |
SFN |
Synonym |
14 3 3 protein sigma, 14-3-3 protein sigma, 1433S_HUMAN, Epithelial cell marker protein 1, Er, HME 1, HME1, MGC143283, Mkrn3, Mme1, |
Backgroud |
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events. |
Swiss Prot |
P31947 |
Gene ID |
2810 |
Subcellular Localization |
Cytoplasm Nucleus Secreted |
Host |
Rabbit |
Clone Type |
Polyclonal Antibody |
Subtype |
IgG |
Protein MW |
28kDa |
Dilution Ratio |
WB 1:500-1000. IHC 1:50-200. |
Immunogen |
A synthetic peptide of human SFN |
Public Immunogen Range |
1-100aa |
Purification Method |
Affinity purification |
Storage Buffer |
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage |
Store at -20℃, avoid freeze/thaw cycles. |
Unit |
Piece |
Cross-Reactivity |
Human Rat Mouse |
Application |
WB IHC |
Specification |
50ul 100ul |