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My CartReactivity：Human,Mouse,Rat
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1676
Host：Rabbit
Qty：
Size：
| Name | Anti-DFFA Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000.IF 1:50-100 |
| Target | DFFA |
| Background | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Swiss Prot | O00273 |
| Gene ID | 1676 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human DFFA |
| Public Immunogen Range | 1-331aa |
| Calculated MW | 29/36kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded breast cancer��hu�� using DFFA antibody at dilution of 1:100 (x100 lens��x200 lens).
Immunofluorescence analysis of HeLa cell using DFFA antibody. Blue: DAPI for nuclear staining.
Western blot analysis with DFFA antibody diluted at 1:1000;Lane: Jurkat H460 HepG2 Mouse brain Rat brain.
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