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My CartReactivity：Rat,Human,Mouse
Application：IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：22059
Host：Rabbit
Qty：
Size：
| Name | Anti-TP53 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:50-200. IF 1:50-200. |
| Target | TP53 |
| Background | This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. |
| Swiss Prot | P02340 |
| Gene ID | 22059 |
| Subcellular Location | Endoplasmic reticulum Mitochondrion Nucleus |
| Immunogen | Recombinant protein of human TP53 |
| Public Immunogen Range | 95-390aa |
| Calculated MW | 44kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with P53 antibody diluted at 1:1000
Immunohistochemistry of paraffin-embedded lung cancer(hu) tissue using P53 antibody at dilution of 1:100 (x40 lens).
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