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My CartReactivity：Mouse,Human
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1984
Host：Rabbit
Qty：
Size：
| Name | Anti-EIF5A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. IF 1:50-200. |
| Target | EIF5A |
| Background | mRNA-binding protein involved in translation elongation. Has an important function at the level of Mrna turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. |
| Swiss Prot | P63241 |
| Gene ID | 1984 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human EIF5A |
| Public Immunogen Range | 1-154aa |
| Calculated MW | 17/20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EIF5A antibody diluted at 1:1000;Lane: HeLa,293T,Raji,22Rv1,HepG2,K562,U251,Mouse liver,Mouse testis.
Immunohistochemistry of paraffin-embedded Human breast cancer with EIF5A antibody diluted at 1:100.
Immunofluorescence analysis of U2OS cells using EIF5A antibody diluted at 1:100.
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