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My CartReactivity：Rat,Mouse,Human
Application：IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：919
Host：Rabbit
Qty：
Size：
| Name | Anti-CD247 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:100-500. IHC 1:50-200. IF 1:50-200. |
| Target | CD247 |
| Background | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Swiss Prot | P20963 |
| Gene ID | 919 |
| Subcellular Location | Membrane Single-pass type I membrane protein |
| Immunogen | Recombinant protein of human CD247 |
| Public Immunogen Range | 50-164aa |
| Calculated MW | 19kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CD247 antibody diluted at 1:1000;Lane: Jurkat,Mouse thymus.
Immunohistochemistry of paraffin-embedded Human esophageal cancer with CD247 antibody diluted at 1:100.
Immunofluorescence analysis of A549 cells using CD247 antibody diluted at 1:100.
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