{{cart_num}}
My CartReactivity：Rat,Mouse,Human
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：6677
Host：Rabbit
Qty：
Size：
| Name | Anti-SPAM1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:50-200. |
| Target | SPAM1 |
| Background | Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. |
| Swiss Prot | P38567 |
| Gene ID | 6677 |
| Subcellular Location | Cell membrane GPI-anchor Lipid-anchor |
| Immunogen | Recombinant protein of human SPAM1 |
| Public Immunogen Range | 100-300aa |
| Calculated MW | 58kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SPAM1 antibody diluted at 1:1000;Lane: Rat testis.
Immunohistochemistry of paraffin-embedded Rat testis with SPAM1 antibody diluted at 1:100.
Immunofluorescence analysis of Rat testis tissue using SPAM1 antibody diluted at 1:100.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
