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My Cart> Antibodies > Primary Antibodies > Anti-DiMethyl-Histone H3-K27 Polyclonal AntibodyReactivity：Mouse,Rat,Human,Other(Wide Range)
Application：IHC,WB,ChIP-seq,ChIP,DB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：8290
Host：Rabbit
Qty：
Size：
| Name | Anti-DiMethyl-Histone H3-K27 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | DB 1:500-1000. WB 1:500-1000. IHC 1:50-200. IF 1:50-200. ChIP 5μg. ChIP-seq 1:20-100. |
| Target | DiMethyl-Histone H3-K27 |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Swiss Prot | Q16695 |
| Gene ID | 8290 |
| Subcellular Location | Chromosome Nucleus |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Public Immunogen Range | 1-100aa |
| Calculated MW | 16kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human Other(Wide Range) |
| Application | ChIP-seq ChIP DB IF WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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