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HPLC
The products were purified using the XDB-C18 separationcolumn (5 μm, 250 × 4.6 mm). Retinol and retinal were detected in a mobile phase consistingof methanol and acetonitrile in a ratio of 95:5 at the detection temperature of 25 ℃ with thedetection wavelength of 352 nm. The mobile phase consisted of methanol, acetonitrile, andisopropanol in a ratio of 3:5:2, and the detection temperature was 40 ℃. The detectionwavelength used for β-carotene was 450 nm.Squalene was detected at 210 nm usingmobile phase methanol at 40 ℃.
References：
Wang X, Xu X, Liu J, Liu Y, Li J, Du G, Lv X, Liu L. Metabolic Engineering of Saccharomyces cerevisiae for Efficient Retinol Synthesis. J Fungi (Basel). 2023 Apr 26;9(5):512. doi: 10.3390/jof9050512. PMID: 37233223; PMCID: PMC10219262.
