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My CartReactivity：Mouse,Rat,Human
Application： IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
Size：
| English Name | Anti-COX6A1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:200-2000.IHC 1:20-200.IF 1:50-200 |
| Target | COX6A1 |
| Background | Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented. |
| Swiss Prot | P12074 |
| Gene ID | 1337 |
| Subcellular Location | Mitochondrion inner membrane |
| Immunogen | Recombinant protein of human COX6A1 |
| Public Immunogen Range | 25-109aa |
| Calculated MW | 12kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded mouse kidney using COX6A1 antibody at dilution of 1:100 (x40 lens).
Western blot analysis with COX6A1 antibody diluted at 1:1000;Lane: A549 MCF7 Mouse kidney Mouse heart Mouse liver Rat liver Rat brain.
Immunohistochemistry of paraffin-embedded rat cerebellum with COX6A1 antibody diluted at 1:100.
Immunofluorescence analysis of C6 cells using COX6A1 antibody diluted at 1:100.
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