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My CartReactivity：Mouse,Rat,Human
Application： IHC,WB,IP,ChIP,IF
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-RNF2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:100-500. IHC 1:50-200. IF 1:50-200. IP 0.5μg-4μg. ChIP 5μg. |
| Target | RNF2 |
| Background | Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. |
| Swiss Prot | Q99496 |
| Gene ID | 6045 |
| Subcellular Location | Chromosome Nucleus |
| Immunogen | Recombinant protein of human RNF2 |
| Public Immunogen Range | 1-336aa |
| Calculated MW | 38kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | ChIP IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with RNF2 antibody diluted at 1:1000;Lane: Jurkat.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human liver cancer with RNF2 antibody diluted at 1:100.
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