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My CartReactivity：Human,Rat,Mouse
Application： WB,IHC,IF
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-MAP1LC3A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000.IHC 1:50-200.IF 1:50-200 |
| Target | MAP1LC3A |
| Background | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. |
| Swiss Prot | Q9H492 |
| Gene ID | 84557 |
| Subcellular Location | Cytoplasm Cytoplasmic vesicle Endomembrane system Lipid-anchor autophagosome autophagosome membrane cytoskeleton |
| Immunogen | A synthetic peptide of human MAP1LC3A |
| Public Immunogen Range | 1-60aa |
| Calculated MW | 14kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MAP1LC3A antibody diluted at 1:1000;Lane: 293T SH-SY5Y A375 Mouse brain Rat brain NIH3T3 C6.
Immunohistochemistry of paraffin-embedded Liver cancerl(hu) using MAP1LC3A antibody at dilution of 1:100 (x100 lens).
Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody.
Immunohistochemistry of paraffin-embedded human colon carcinoma with MAP1LC3A antibody diluted at 1:100.
Immunofluorescence analysis of 293T Treated with Chloroquine and 293T cells using MAP1LC3A antibody diluted at 1:100.
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