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My CartReactivity：Human,Rat,Mouse
Application： IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-ANPEP Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:50-100. |
| Target | ANPEP |
| Background | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. |
| Swiss Prot | P15144 |
| Gene ID | 290 |
| Subcellular Location | Cell membrane Cytoplasm Single-pass type II membrane protein cytosol |
| Immunogen | Recombinant protein of human ANPEP |
| Public Immunogen Range | 668-967aa |
| Calculated MW | 110kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ANPEP antibody diluted at 1:1000;Lane: Mouse kidney Rat kidney.
Immunohistochemistry of paraffin-embedded human placenta with ANPEP antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using ANPEP antibody diluted at 1:100.
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