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My CartReactivity：Rat,Human,Mouse
Application： IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-PSMA5 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:50-200. |
| Target | PSMA5 |
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. |
| Swiss Prot | P28066 |
| Gene ID | 5686 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human PSMA5 |
| Public Immunogen Range | 1-241aa |
| Calculated MW | 26kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PSMA5 antibody diluted at 1:1000;Lane: HeLa 293T Jurkat U-251MG Mouse liver Mouse brain Mouse spleen Rat brain Rat spleen.
Immunohistochemistry of paraffin-embedded Pancreatic cancer(hu) using PSMA5 antibody at dilution of 1:100 (x100 lens,x200 lens).
Immunofluorescence analysis of U20S cell using PSMA5 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon carcinoma with PSMA5 antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using PSMA5 antibody diluted at 1:100.
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