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My CartReactivity：Mouse,Rat,Human
Application： IHC,IF,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-ATP5B Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:10000-30000. IHC 1:50-200. IF 1:50-200. |
| Target | ATP5B |
| Background | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. |
| Swiss Prot | P06576 |
| Gene ID | 506 |
| Subcellular Location | Mitochondrion Mitochondrion inner membrane |
| Immunogen | Recombinant protein of human ATP5B |
| Public Immunogen Range | 230-529aa |
| Calculated MW | 57kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | IHC IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ATP5B antibody diluted at 1:1000
Immunohistochemistry of paraffin-embedded human liver injury using ATP5B antibody at dilution of 1:100 (x40 lens).
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