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My CartReactivity：Rat,Human,Mouse
Application： IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-COL1A2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-200. IF 1:25-200. |
| Target | COL1A2 |
| Background | This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
| Swiss Prot | P08123 |
| Gene ID | 1278 |
| Subcellular Location | Extracellular matrix Secreted |
| Immunogen | A synthetic peptide of human COL1A2 |
| Public Immunogen Range | 501-600aa |
| Calculated MW | 129kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with COL1A2 antibody diluted at 1:1000;Lane: A375.
Immunohistochemistry of paraffin-embedded Human placenta with COL1A2 antibody diluted at 1:100
Immunofluorescence analysis of A-431 cells using COL1A2 antibody diluted at 1:100
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