English
中文
CAS:87915-38-6
Appearance:Blue crystalline powder
Storage:RT.
蓝色葡聚糖2000是用于检测过滤柱装填是否合格。
蓝色葡聚糖 2000 Dextran Blue 2000
CAS Number: 87915-38-6
Storage Temperature: Room Temperature
Product Description :
The most common use for Blue Dextran is in the determination of the void volume in gel filtration chromatography columns. It is typically used at 1 mg/ml to 2 mg/ml in a buffer such as 50 mM Tris-HCl, pH 7.5 containing 100 mM KCl. The volume of the Blue Dextran sample should be 1% to no more than 3% of the column's bed volume. The shape of the Blue Dextran band as it elutes through the column can also be used to assess the column packing and resolution.
Blue Dextran has also been used as a spectrophotometric substrate for dextranase. Dextranase activity is measured by the increase in absorbance at 610 nm.
The affinity of the Reactive Blue 2 Dye in Blue dextran for certain proteins has been exploited in protein purification methods. When bound to a protein the Blue Dextran-protein complex will be eluted in the void volume of a sephadex gel filtration column. This allows the bound protein to be easily separated from other proteins of similar molecular weight. The binding of blue dextrans to proteins is dependent on pH and ionic strength.
Storage/Stability :
This product is given a shelf-life of five years from the date of manufacture. The solubility of Blue Dextran has been tested at 50 mg/ml in water. Dextran can be hydrolyzed by strong acids at high temperatures. The terminal reducing end group of dextran can be oxidized in alkaline solutions.Solutions should be prepared fresh when ever possible. Dextran is susceptible to microbial degradation. Solutions should be stored refrigerated.
Precautions and Disclaimer :
For Laboratory Use Only. Not for drug, household or other uses.
相关产品：
D7250 Dextran 20000 葡聚糖2万 右旋糖酐20
D8240 DextranT-10 葡聚糖T-10
D8250 Dextran T-40 葡聚糖 T-40
D8251 Dextran T-40 葡聚糖 T-40 分子量 4 万
D8260 Dextran T-70 葡聚糖 T-70
D8270 Dextran T-500 葡聚糖T-500
D8320 Dextran Sulfate 硫酸葡聚糖钠 （MW 500,000）
D8321 Dextran sulfate sodium salt 硫酸葡聚糖钠 （MW 5000）
D8290 Dextran Blue 2000 蓝色葡聚糖 2000
备注：产品信息可能会有优化升级。请以实际标签信息为准。
