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Determination of Significance：
Sugar is one of the important components of plant, also is the main material of metabolism and storage. Total sugar refers to the reductive monosaccharide in the sample and the sucrose, maltose and starch which can be hydrolyzed or partially hydrolyzed to glucose by this test method.
Measurement Principle：
Anthrone colorimetric method can be used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, simple and quick, and suitable for the determination of trace samples.
Self Provided：
Spectrophotometer, water bath, 1 mL glass cuvette, transferpettor, concentrated sulfuric acid, mortar/homogenizer, distilled water.
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