Determination of Significance:
Peroxidase (POD, EC 1.11.1.7) widely exists in animals, plants and microorganisms. It can catalyze the oxidation of phenols and amines by hydrogen peroxide, and has the dual effect of eliminating toxicity of hydrogen peroxide, phenols and amines. In the presence of hydrogen peroxide, POD can catalyze H2O2 oxidize specific substrates to produce one substance which has a absorption at 470 nm.
Measurement Principle:
Peroxidase (POD, EC 1.11.1.7) widely exists in animals, plants and microorganisms. It can catalyze the oxidation of phenols and amines by hydrogen peroxide, and has the dual effect of eliminating toxicity of hydrogen peroxide, phenols and amines. In the presence of hydrogen peroxide, POD can catalyze H2O2 oxidize specific substrates to produce one substance which has a absorption at 470 nm.
Self Provided:
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogeniser, ice and distilled water.
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