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Determination of Significance：
Soil Polyphenol Oxidase (S-PPO) mainly comes from the decomposition and release of soil microorganisms, plant root secretions as well as animals and plants residues. S-PPO catalyzes the oxidation of aromatic compounds into quinone in soil. Quinone reacts with proteins, amino acids, sugars, minerals and other substances in soil to generate organic matters and pigments. Therefore, S-PPO enables the soil to complete the cycle of aromatic compounds and be used for soil environmental restoration.
Measurement Principle：
S-PPO is able to catalyzes the pyrogallol to produce purple gallic acid, which has characteristic absorption peak at 430 nm.
Self Provided：
Table centrifuge, water-bath, transferpettor, spectrophotometer, 1 ml glass cuvette, Ether (express delivery is not allowed), ice and distilled water.
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