Determination of Significance:
Soil phosphatase is an enzyme which catalyze soil organic phosphate mineralization, the activity influence directly the decomposition and transformation of organic phosphate and its bio-availability. The activity is the indicator of evaluating the direction and intensity of soil phosphorus bio-transformation. Soil phosphatase is influenced by the content of carbon, nitrogen, available phosphorus in the soil and pH. Soil phosphatase is divided into three types: acidic, neutral and alkaline phosphatase according to the optimum pH.
Measurement Principle:
In acidic condition, soil acid phosphatase (S-ACP) can hydrolyze disodium phenyl phosphate to phenol and disodium hydrogen phosphate. The activity of S-ACP can be calculated by measuring the amount of phenol produced.
Self Provided:
Spectrophotometer, Water bath/constant temperature incubator, centrifuge, transferpettor, table centrifuge, 1 mL glass cuvette, analytical balance, toluene, alcohol, ice and distilled water.
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