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Determination of Significance：
Soil β-glucosidase (S-β-GC) can catalyze the hydrolysis of glycoside bonds between aryl or hydroxyl groups and glycosylated atomic groups to generate glucose. It is an important component of cellulose decomposition enzyme system and has important physiological functions in the carbohydrate metabolism of soil microorganisms.
Measurement Principle：
S-β-GC can catalyze the p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The product is slightly yellow and has characteristic of absorption at 400 nm.
Self Provided：
Table centrifuge, water-bath, transferpettor, spectrophotometer, 1 mL glass cuvette, ice, 30 mesh sieve (or smaller), methylbenzene (express delivery is not allowed) and distilled water.
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