Determination of Significance:
Ascorbate Peroxidase (APX) is an important antioxidase of plant scavenging reactive oxygen, also is one key enzyme of ascorbic acid metabolism. APX has a variety of isozymes located in chloroplast, cytoplasm, mitochondria, peroxides and glyoxylate, peroxisome and thylakoid membrane respectively. APX is the main consumer of plant AsA, which catalyzes the oxidation of AsA by H2O2. The activity of APX directly affects the content of ASA, and there is a negative correlation between APX and ASA.
Measurement Principle:
APX catalyzes the oxidation of ASA by H2O2. In this kit, the activity of APX is calculate by the oxidize rate of AsA.
Self Provided:
Refrigerated centrifuge, spectrophotometer, 1 mL quartz cuvette, transferpettor, mortar/ homogenizer, ice and distilled water.
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