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Determination of Significance：
Reducing sugar is widely found in animals, plants, microorganisms and cultured cells. Reducing sugars in plants mainly include glucose, fructose and maltose, which are the most common monosaccharides and disaccharides. Glucose and fructose are not only the main substrates of respiration, but also the substrates for further synthesis of sucrose, starch and cellulose.
Measurement Principle：
Heating can promote the formation of brownish red amino compound from 3,5-Dinitrosalicylic acid solution and reducing sugar in alkaline solution. This brownish red amino compound has a characteristic absorption peak at 540 nm. Within a certain concentration range, the content of reducing sugar has a linear relationship with the absorbance at 540 nm. According to the standard curve, the amount of reducing sugar in the sample can be calculated.
Self Provided：
Spectrophotometer, water-bath, table centrifuge, transferpettor, sonicator, 1 mL glass cuvette, mortar/ homogenizer, distilled water.
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