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Determination of Significance：
Soil Saccharase (S-SC) can hydrolyze saccharose into corresponding monosaccharides and can be absorbed by the body. The enzymatic product is an important index for evaluating soil fertility, which is closely related to the content of organic matter, nitrogen and phosphorus in soil, the number of microorganisms and soil respiration intensity.
Measurement Principle：
S-SC catalyzes saccharose to form reduced sugar. The reduced sugar can react with 3,5- dinitrosalicylic acid to form a brownish red amino complex which can be detected by colorimetric assay at 540 nm. In this kit, the soil saccharase activity can be determined by measuring the color depth at 540 nm indirectly.
Self Provided：
Spectrophotometer, water bath, desk centrifuge, transferpettor, 1 mL glass cuvette, 30 mesh sieve (or smaller), methylbenzene (express delivery not allowed), ice and distilled water.
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