Hydroxyproline(HYP) Content Assay Kit
Cat.No：BC0250 Solarbio
Detection Method：Spectrophotometer
Storage：Store at 2-8℃，6 months
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Your Position: Home
> Biochemical Assay Kits > Nitrogen Metabolism Series > Amino Acid Metabolism Series > Hydroxyproline(HYP) Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Hydroxyproline(HYP) Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
HYP is one of the main components of collagen in the body. Most of the collagen is distributed in the skin, tendon, cartilage and blood vessels et al. Therefore, the content of HYP is an important index reflecting the metabolism and fibrosis degree of collagen tissue.
Measurement Principle：
The sample is hydrolyzed to produce free HYP, which is further oxidized by chloramine T. The oxidized product reacted with p-Dimethylaminobenzaldehyde to produce red compound with characteristic absorption peak at 560 nm. The content of HYP can be calculated by measuring the absorption value of sample hydrolysate at 560 nm.
Self Provided：
Scales, oven, glass tube, centrifuge, water bath, spectrophotometer, 1 mL glass cuvette, 6 mol/L HCl and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Hydroxyproline(HYP) Content Assay Kit
Related Products
Sorry, there is no more information.
Related Paper
DT-109 ameliorates nonalcoholic steatohepatitis in nonhuman primates.
Click to check >>Author：Qu P, Rom O, Li K, Jia L, Gao X, Liu Z, Ding S, Zhao M, Wang H, Chen S, Xiong X, Zhao Y, Xue C, Chu C, Wen B, Finney AC, Zheng Z, Cao W, Zhao J, Bai L, Zhao S, Sun D, Zeng R, Lin J, Liu W, Zheng L, Zhang J, Liu E, Chen YE
IF：31.3730
Publish_to：Cell Metabolism
PMID：37040763
Inhaled mRNA nanoformulation with biogenic ribosomal protein reverses established pulmonary fibrosis in a bleomycin-induced murine model
Click to check >>Author：张蕊
IF：30.8490
Publish_to：Advanced Materials
PMID：35146813
Iron oxide nanoparticles augment the intercellular mitochondrial transfer?mediated therapy
Click to check >>Author：黄婷
IF：14.1360
Publish_to：
PMID：34586849
Biomimetic Antithrombotic Tissue-Engineered Vascular Grafts for Converting Cholesterol and Free Radicals into Nitric Oxide
Click to check >>Author：Dongcheng Yang, Yanzhao Li, Ju Tan, Wenya Li, Zilu Xu, Jianhua Xu, Chunli Hou, Jingting Zhou, Gang Li, Mingcan Yang, Yong Liu, Qiaorui Tang, Xiaohan Zhang, Wen Zeng, Xuli Feng, Chuhong Zhu
IF：11.0920
Publish_to：Advanced Healthcare Materials
PMID：37154485
Novel strategy of combined interstitial macrophage depletion with intravenous targeted therapy to ameliorate pulmonary fibrosis
Click to check >>Author：Zhongxian Li, Qiang Zhang, Jiawei Xiang, Mingyuan Zhao, Yuan Meng, Xuhao Hu, Tingting Li, Yifeng Nie, Huizhen Sun, Tun Yan, Zhuo Ao, Dong Han
IF：10.7610
Publish_to：Materials Today Bio
PMID：37214554
Autophagy-Interfering Nanoboat Drifting along CD44–Golgi–ER Flow as RNAi Therapeutics for Hepatic Fibrosis
Click to check >>Author：Yashi Wang, Lin Xiong, Ziyan Dong, Kaixin Ran, Wenjing Bai, Ziyi Mo, Kexin Huang, Yunxia Ye, Yuan Tao, Sheng Yin, Man Li, Qin He
IF：9.5000
Publish_to：ACS Applied Materials & Interfaces
PMID：37290012
FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosis
Click to check >>Author：Hailong Li, Jinhe Li, Yayue Hu, Ruotong Zhang, Xiaoting Gu, Yiying Wei, Shanshan Zhang, Xuefen Chen, Luqing Wei, Xiaohe Li, Songtao Gu, Jin Jin, Hui Huang, Honggang Zhou, Cheng Yang
IF：9.2000
Publish_to：International Journal of Biological Sciences
PMID：37416778
Mesoporous polydopamine based biominetic nanodrug ameliorates liver fibrosis via antioxidation and TGF-β/SMADS pathway
Click to check >>Author：Zihui Tang, Xiaojuan Li, Le Tian, Yuhao Sun, Xinyan Zhu, Fei Liu
IF：8.2000
Publish_to：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
PMID：37482153
Insight into the composite assembly process,nanofibril structure and stability of undenatured type II collagen in the presence of different types of nanocelluloses
Click to check >>Author：Yuan Wang, Liang Zhang, Like Mao, Fang Yuan, Jinfang Liu, Yanxiang Gao
IF：8.0250
Publish_to：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
PMID：37085069
A chondrogenesis induction system based on functionalized hyaluronic acid hydrogel sequentially promoting hMSC proliferation, condensation, differentiation and matrix deposition
Click to check >>Author：滕彬宏
IF：7.2400
Publish_to：
PMID：33444801
The engineered CRISPR-Mb2Cas12a variant enables sensitive and fast nucleic acid-based pathogens diagnostics in the field
Click to check >>Author：Zihui Tang, Bowen Lin, Wei Li, Xiaojuan Li, Fei Liu, Xinyan Zhu
IF：4.8000
Publish_to：CELLULAR SIGNALLING
PMID：37290675
Technical Q&A
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.
溶液中钙离子和磷酸根离子会对结果有影响吗 2023-03-11 21:33:19
Administrator理论上是不受影响的。
对组织的要求是什么？
2022-09-21 16:21:20
Administrator试剂盒没有种属限制，新鲜组织和冻存组织都可以检测，但冻存组织保存时间不要过长，一般-20℃保存1个月，-80℃保存6个月