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Determination of Significance：
HYP is one of the main components of collagen in the body. Most of the collagen is distributed in the skin, tendon, cartilage and blood vessels et al. Therefore, the content of HYP is an important index reflecting the metabolism and fibrosis degree of collagen tissue.
Measurement Principle：
The sample is hydrolyzed to produce free HYP, which is further oxidized by chloramine T. The oxidized product reacted with p-Dimethylaminobenzaldehyde to produce red compound with characteristic absorption peak at 560 nm. The content of HYP can be calculated by measuring the absorption value of sample hydrolysate at 560 nm.
Self Provided：
Scales, oven, glass tube, centrifuge, water bath, spectrophotometer, 1 mL glass cuvette, 6 mol/L HCl and distilled water.
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溶液中钙离子和磷酸根离子会对结果有影响吗2023-03-11 21:33:19
Administrator理论上是不受影响的。
对组织的要求是什么？
2022-09-21 16:21:20
Administrator试剂盒没有种属限制，新鲜组织和冻存组织都可以检测，但冻存组织保存时间不要过长，一般-20℃保存1个月，-80℃保存6个月