Storage:Store at 2-8℃,6 months
Determination of Significance:
Soil phosphatase is an enzyme which catalyzes soil organic phosphate mineralization, the activity level influence the decomposition and transformation of organic phosphate and its bio-availability directly, which is the index of evaluating the direction and intensity of soil phosphorus bio-transformation. Soil phosphatase is influenced by the content of carbon, nitrogen, available phosphorus in the soil and pH, its divided into three types: acidic, neutral and alkaline according to the optimum pH.
Measurement Principle:
In alkaline condition, soil alkaline phosphatase (S-AKP/ALP) can catalyzes disodium phenyl phosphate to form phenol and disodium hydrogen phosphate, the activity of S-AKP/ALP can be calculated by detecting the content of phenol.
Self Provided:
Spectrophotometer, Water bath/constant temperature incubator, desk centrifuge, transferpettor, 1 mL glass cuvette, analytical balance, mythylbenzene (express delivery not allowed), alcohol, ice and distilled water.
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