Determination of Significance:
Trehalose is found in a large number of organisms, including bacteria, algae, yeast, plants, insects, and other invertebrates. As trehalose has unique biological characteristics different from other carbohydrates, it can protect organisms' cell proteins, fats, sugars, nucleic acids and other groups in harsh environments such as drought, high temperature, dehydration, freezing, high osmotic pressure and toxic substances points are not impaired.
Measurement Principle:
The measurement method is anthrone colorimetric method. It has the advantages of high sensitivity, simple and fast, and suitable for the determination of trace samples. However, the anthrone colorimetric method also has certain defects. If the sample contains soluble sugar, it will affect the determination. This kit is recommended for determination of samples that do not contain soluble sugar other than trehalose.
Self Provided:
Spectrophotometer, water bath, adjustable transferpettor, Sonicator,mortar/homogenizer, centrifuge, ice, 1 mL glass cuvette, concentrated sulfuric (H2SO4) acid and distilled water.
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