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Determination of Significance：
Fluorescein diacetate (FDA) hydrolysis is one of the most important biological indicators in the study of soil quality, which can reflect the activity of soil microbial, the change of soil quality and the transformation of organic matter in ecosystem.
Measurement Principle：
FDA is a colorless compound, which can be hydrolyzed by many soil enzymes in the medium. After dehydration reaction, fluorescein is the final product of enzymatic hydrolysis. The fluorescein is stable and not easy to be decomposed, and has a strong absorption peak at 490 nm. The activity of FDA hydrolase can be calculated by detecting the change of absorption value at 490 nm.
Self Provided：
Balance, tabletop centrifuge, spectrophotometer, 1 mL glass cuvette, constant temperature water bath, acetone, 30-50 mesh sieve (or smaller).
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