Determination of Significance:
Fatty acid synthase (FAS) is an important enzyme in the synthesis of long-chain saturated fatty acids.
Measurement Principle:
It can catalyze malonyl coenzyme A, acetyl coenzyme A and NADPH to produce long chain fatty acids and NADP+. NADPH has a characteristic absorption peak at 340nm. The activity of FAS can be calculated by measuring the decreasing rate of absorbance at 340nm.
Self Provided:
Ultraviolet Spectrophotometer, Water-Bath/Constant Temperature Incubator, Desk Centrifuge, Pipette, 1 mL Quartz Cuvette, Mortar/ Homogenizer/Cell Ultrasonic Crusher, Ice, Distilled Water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
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3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.