Neutral Invertase(NI) Activity Assay Kit

Determination of Significance：

Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, which is one of the key enzymes in sucrose metabolism of higher plants. According to the optimal pH, Ivr can be divided into acid invertase (AI) and neutral invertase (NI). NI mainly exists in the cytoplasm and is responsible for decomposing sucrose into fructose and glucose.

Measurement Principle：

NI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has a characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to NI activity. The activity of NI is calculated by the increasing rate of light absorption.

Self Provided：

NI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has a characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to NI activity. The activity of NI is calculated by the increasing rate of light absorption.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.