Determination of Significance:
Sucrose is the main form of transport of photosynthetic products from source (leaf, etc.) to "sink" organs. Sucrose synthetase (SS, EC 2.4.1.13) catalyzes the synthesis of sucrose from free fructose and glucose in plants.
Measurement Principle:
SS catalyzes the reaction between free fructose and glucose donor UDPG to generate sucrose, and the reaction between sucrose and resorcinol can show color changes. There is a characteristic absorption peak at 480 nm, and the enzyme activity of SS is proportional to the color.
Self Provided:
Spectrophotometer, water-bath, table centrifuge, adjustable pipette, 1 mL glass cuvette, ice, mortar/homogenizer and distilled water.
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